DSPath NGS Multiplex PCR Master Mix #K030-B tNGS library preparation

【Product Description】

DSPath NGS Multiplex PCR Master Mix Ⅱ is a multiplex PCR premix for NGS (high throughput sequencing) library preparation, which is applicable for pathogenic microorganisms detection. This product contains various components required for multiplex PCR reactions (except primers and templates). The hotstart high-fidelity DNA polymerase has the characteristics of low mismatch rate and good specificity, and is suitable for Panel multiplicities up to 1000. The optimized buffer system reduces the inhibition of multiple sample interferences on amplification, and is suitable for DNA amplification of various samples such as blood, nose/throat swab, virus culture, etc. DSPath NGS Multiplex PCR Master Mix can quickly complete the amplification library.

【Components】

【Storage Condition】

Store at -20°C.

【Application】

pathogenic microorganism tNGS; cancer gene tNGS; genetic disease tNGS; scientific research

Features

Excellent amplification performance: low mismatch rate and high specificity, capable of conducting super multiple amplification to construct high-quality DNA libraries.

Easy to use: all-in-one master mix, reducing operation and saving time in library preparation.

Wide applicability: compatible with various samples such as blood, nasal / throat swabs, viral cultures, and is widely used in pathogenic microorganism detection, cancer gene detection, scientific research, and other fields.

Validation Data

The DNA pathogen library constructed using GDSBio DSPath NGS Multiplex PCR Master Mix was tested using 480-plex pathogen panel, with a target read detection rate of 65 %, significantly higher than other similar products; the ratio of primer dimer reads is as low as 2.5 %, significantly lower than other similar products; at the same time, the homogeneity of the library is good.

Fig. 1 Detection rate of target reads

Fig.2 Proportion of primer dimer

Fig. 3 Homogeneity of the Library

Founded in 2005, located in the Science City of Guangzhou, China, Guangzhou Dongsheng Biotech Co., Ltd (GDSBio) is a high-tech enterprise focusing on R&D, production and sales of high-quality life science products and in vitro diagnostic reagents.

GDSBio has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, NGS library preparation, nucleic acid electrophoresis, nucleic acid purification, etc.It has developed reagents for molecular biology research and diagnosis, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.

Supply Mode: retail/bulk/custom/OEM

With strict and perfect standards, we focus on each product to ensure that every product delivered is in perfect condition.

We provide professional and comprehensive diagnostic and medical test solutions. And we work with clinical experts to continuously improve technology in practice so as to provide more accurate test data for clinical diagnosis.

Since 2010, when GDSBio was certified with ISO9001 and ISO13485 standards in sequence, it has opened up overseas markets. Over the past 18 years, our customers have spread all over the world. With stable and excellent quality, the products have won a large number of loyal customers.

GDSBio's international resource links and multi-dimensional biological research methods help it stand at the forefront of the industry. To interpret the world with high-end technology, insight into the biological mystery.

ISO 13485 certificate

ISO 9001 certificate