【Product Name】: Alpaca Rapid Aqueous Adjuvant
【Main Ingredients】: Nano water-based adjuvant, polymer materials.
【Characteristics】: White transparent emulsion.
【Function and Purpose】: The reagent itself is a water-soluble adjuvant complex. There is
no need for the complicated emulsification process of Freund's
adjuvant when in use. The antigen and adjuvant only need to be
mixed simply to immunize animals. Can be used via intramuscular or
subcutaneous immunization routes. It has good broad-spectrum
antibody production, high antibody titers, and high antibody
affinity. By reducing the number of immunizations and lowering the
antigen dose per immunization, total antigen usage is saved,
greatly shortening the antibody production cycle, far superior to
industry standards.
【Usage and Dosage】:
1.Dilute the antigen with physiological saline to twice the final
concentration (prepare according to an antigen dose of 500 μl per
injection). Recommended antigen doses:
•(1) For subunit protein antigens with weak immunogenicity, 600 μg
per injection.
•(2) For inactivated whole virus or whole bacteria and virus-like
particle antigens with strong immunogenicity, 100 μg per injection.
•(3) For small molecule antigens conjugated to carrier proteins,
400 μg per injection. (Actual dosage should be based on preliminary
data and company experience.)
2.Thoroughly mix the adjuvant (it is recommended to use a syringe
to mix repeatedly), and under sterile conditions, take the required
amount (500 μl per injection) and mix quickly with the antigen in a
1:1 volume ratio. (Slight precipitation of the adjuvant is normal,
ensure thorough mixing with the antigen and inject promptly.)
3.Inject subcutaneously near the cervical lymph nodes on both
sides, in two points per side, administering 1 ml per alpaca. (The
injection method can follow the company's routine operations.)
•(1) Slight precipitation of the adjuvant after mixing with the
antigen is normal. Ensure thorough mixing before drawing into the
syringe and inject promptly.
•(2) Alternatively, subcutaneous or intradermal injection can be
chosen based on experimental habits.
4.On the 10th-14th day, administer a booster injection with the
same dose and method (the timing of the second injection should be
determined based on the type of antigen). Note: Always prepare and
use the adjuvant and antigen freshly mixed, injecting at the same
site as the initial immunization. (Follow the company’s actual
immunization schedule for booster immunizations.)
5.IgG can be detected on the 21st day, and a small blood sample can
be collected on the 28th day for ELISA testing. (The above is a
predicted schedule; actual data conclusions should follow the
experimental results.) The antibody titer can reach its peak. Then,
whole blood can be collected, or antigen challenge immunization and
spleen cell fusion can be performed following standard methods.
6.If the titer on the 28th day is below expectations, an additional
booster shot with the same dose and method can be given around the
28th day. Titer testing can be performed on the 35th-42nd days,
generally reaching peak antibody titer. Subsequently, whole blood
collection or antigen challenge immunization and spleen cell fusion
can proceed following standard methods.
【Storage and Shelf Life】: Store at 4-8°C, aseptically remove, shelf life is two years.
【Manufacturer】: Wuhan Melon New Materials Co., Ltd
